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A leader intron and 115‐bp promoter region necessary for expression of the carnation S ‐adenosylmethionine decarboxylase gene in the pollen of transgenic tobacco
Author(s) -
Kim Young Jin,
Lee Sun Hi,
Park Ky Young
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.11.005
Subject(s) - biology , pollen , petal , gene , stamen , gene expression , transgene , regulatory sequence , microbiology and biotechnology , intron , genetics , botany
The expression of CSDC9 encoding S ‐adenosylmethionine decarboxylase (SAMDC) is developmentally and spatially regulated in carnation. To examine the regulation of the SAMDC gene, we analyzed the spatial expression of CSDC9 with a 5′‐flanking β‐glucuronidase fusion in transgenic tobacco plants. GUS was strongly expressed in flower, pollen, stem and vein of cotyledons. Expression in both anther and stigma was under developmental control; analysis of a series of mutants with deletions of the 5′‐flanking region demonstrated differential activation in petal, anther, stigma and pollen grains. All the major cis ‐regulatory elements required for pollen‐specific transcription were located in the upstream region between −273 and −158. This region contains four putative elements related to gibberellin induction (pyrimidine boxes, CC and CCTTTT) and pollen‐specific expression (GTGA and AGAAA). In addition, the first 5′‐leader intron was necessary for tissue‐specific expression.