Premium
Volatile anesthetic modulation of oligomerization equilibria in a hexameric model peptide
Author(s) -
Ghirlanda Giovanna,
Hilcove Simon A.,
Pidikiti Ravindernath,
Johansson Jonas S.,
Lear James D.,
DeGrado William F.,
Eckenhoff Roderic G.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.087
Subject(s) - chemistry , oligomer , anesthetic , halothane , peptide , biophysics , residue (chemistry) , volatile anesthetic , molecule , stereochemistry , biochemistry , isoflurane , organic chemistry , biology , anesthesia , medicine
To determine if occupancy of interfacial pockets in oligomeric proteins by volatile anesthetic molecules can allosterically regulate oligomerization equilibria, variants of a three‐helix bundle peptide able to form higher oligomers were studied with analytical ultracentrifugation, hydrogen exchange and modeling. Halothane shifted the oligomerization equilibria towards the oligomer only in a mutation predicted to create sufficient volume in the hexameric pocket. Other mutations at this residue, predicted to create a too small or too polar pocket, were unaffected by halothane. Inhaled anesthetic modulation of oligomerization interactions is a novel and potentially generalizable biophysical basis for some anesthetic actions.