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Enhancing the first enzymatic step in the histidine biosynthesis pathway increases the free histidine pool and nickel tolerance in Arabidopsis thaliana
Author(s) -
Wycisk Katharina,
Kim Eugene J.,
Schroeder Julian I.,
Krämer Ute
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.086
Subject(s) - histidine , arabidopsis thaliana , biochemistry , enzyme , biosynthesis , biology , endogeny , arabidopsis , chemistry , wild type , mutant , gene
Naturally selected nickel (Ni) tolerance in Alyssum lesbiacum has been proposed to involve constitutively high levels of endogenous free histidine. Transgenic Arabidopsis thaliana expressing a Salmonella typhimurium ATP phosphoribosyl transferase enzyme ( St HisG) resistant to feedback inhibition by histidine contained approximately 2‐fold higher histidine concentrations than wild type plants. Under exposure to a toxic Ni concentration, biomass production in St HisG expressing lines was between 14‐ and 40‐fold higher than in wild‐type plants. This suggested that enhancing the first step in the histidine biosynthesis pathway is sufficient to increase the endogenous free histidine pool and Ni tolerance in A. thaliana .