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Atg21p is essential for macropexophagy and microautophagy in the yeast Hansenula polymorpha
Author(s) -
Leão-Helder Adria.,
Krikken Arjen M.,
Gellissen Gerd,
van der Klei Ida J.,
Veenhuis Marten,
Kiel Jan A.K.W.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.055
Subject(s) - vacuole , cytosol , peroxisome , yeast , organelle , biochemistry , microbiology and biotechnology , gene , proteolysis , biology , chemistry , enzyme , cytoplasm
ATG genes are required for autophagy‐related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp‐Atg21p, fused to eGFP, had a dual location in the cytosol and in peri‐vacuolar dots. We demonstrate that Hp‐Atg21p is essential for two separate modes of peroxisome degradation, namely glucose‐induced macropexophagy and nitrogen limitation‐induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.