Premium
Phorbol ester‐induced differentiation of L6 myogenic cells involves phospholipase D activation
Author(s) -
Komati Hiba,
Minasi Alessandra,
Naro Fabio,
Lagarde Michel,
Prigent Annie-France,
Adamo Sergio,
Némoz Georges
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.036
Subject(s) - phospholipase d , activator (genetics) , downregulation and upregulation , cellular differentiation , chemistry , myocyte , protein kinase c , microbiology and biotechnology , phospholipase , phospholipase c , signal transduction , biochemistry , biology , enzyme , receptor , gene
TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA‐induced differentiation was inhibited by 1‐butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation.