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Analysis of estrogen receptor α signaling complex at the plasma membrane
Author(s) -
Azuma Kotaro,
Horie Kuniko,
Inoue Satoshi,
Ouchi Yasuyoshi,
Sakai Ryuichi
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.025
Subject(s) - estrogen receptor , estrogen , chemistry , microbiology and biotechnology , receptor , signal transduction , endocrinology , medicine , biochemistry , biology , cancer , breast cancer
There is accumulating evidence that the estrogen receptor (ER) can transduce specific signals at the plasma membrane. We tried to clarify the biological function of ER as a signaling molecule by identifying proteins that interact with the membrane‐localized ER. The activation function 1 and 2 (AF‐1 and AF‐2) domains of ERα with or without the membrane‐targeting sequence were stably expressed in the breast cancer cell line, MCF‐7. The level of tyrosine phosphorylation of AF‐2 was significantly elevated by the membrane localization. By mass‐spectrometry analysis, α‐ and β‐tubulins and heat shock protein 70 were identified as the AF‐1‐associated proteins. Of these, tubulins are associated only with membrane‐targeted AF‐1.