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Ethanol acts as a potent agent sensitizing colon cancer cells to the TRAIL‐induced apoptosis
Author(s) -
Vaculová Alena,
Hofmanová Jiřina,
Souček Karel,
Anděra Ladislav,
Kozubı́k Alois
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.013
Subject(s) - apoptosis , poly adp ribose polymerase , chemistry , in vitro , programmed cell death , caspase , in vivo , colorectal cancer , cancer cell , ethanol , cleavage (geology) , microbiology and biotechnology , cancer research , mitochondrion , biochemistry , polymerase , biology , cancer , enzyme , paleontology , genetics , fracture (geology)
Identification of mechanisms of modulation of the TNF‐related apoptosis‐inducing ligand (TRAIL)‐induced apoptosis is important for its potential use in anticancer therapy. Ethanol can induce cell death in vitro and in vivo by different signalling pathways. Its effect in combination with death ligands is unknown. We investigated how ethanol modulates the effects of TRAIL in colon cancer cells. After combined TRAIL and ethanol treatment, a potentiation of caspase‐8, ‐9, ‐3 activation, a proapoptotic Bid protein cleavage, a decrease of mitochondrial membrane potential, a complete poly(ADP)ribose polymerase cleavage, and disappearance of antiapoptotic Mcl‐1 protein were demonstrated. Ethanol acts as a potent agent sensitizing colon cancer cells to TRAIL‐induced apoptosis.