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Conserved transcriptional regulation of a cone phototransduction gene in vertebrates
Author(s) -
Viczian A.S.,
Verardo M.,
Zuber M.E.,
Knox B.E.,
Farber D.B.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.10.008
Subject(s) - visual phototransduction , xenopus , biology , transcription (linguistics) , green fluorescent protein , complementary dna , gene , microbiology and biotechnology , reporter gene , phosphodiesterase , transfection , transgene , gene expression , genetics , biochemistry , retinal , enzyme , linguistics , philosophy
cGMP‐phosphodiesterase (PDE) is a key component in visual phototransduction. Rod and cone photoreceptors each produce their unique cGMP‐PDE subunits. The α ′ catalytic subunits are believed to be cone‐specific. In this study, we report that transfection of the −132 to +139 sequence in the upstream region of the human α ′ ‐PDE gene fused to luciferase cDNA gives the highest level of reporter gene transcription in cultured retinoblastoma Y79 cells. Transgenic Xenopus laevis carrying this sequence fused to green fluorescent protein (GFP) expressed GFP in cones, suggesting a conserved regulatory mechanism for α ′ ‐PDE transcription in both human and frog.