Premium
The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl‐aminonucleoside synthetase
Author(s) -
Ángel Rubio Miguel,
Barrado Patricia,
Carlos Espinosa Juan,
Jiménez Antonio,
Lobato Marı́a Fernández
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.087
Subject(s) - puromycin , gene cluster , streptomyces , biology , gene , biochemistry , biosynthesis , deoxyadenosine , escherichia coli , plasmid , microbiology and biotechnology , protein biosynthesis , bacteria , genetics , dna
The pur6 gene of the puromycin biosynthetic gene ( pur ) cluster from Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking l ‐tyrosine to both 3 ′ ‐amino‐3 ′ ‐deoxyadenosine and N 6 , N 6 ‐dimethyl‐3 ′ ‐amino‐3 ′ ‐deoxyadenosine with a peptide‐like bond. Identical reactions were performed by cell lysates from Streptomyces lividans or Escherichia coli transformants that expressed pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and O ‐demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl‐aminonucleoside synthetase of the puromycin biosynthetic pathway.