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ETS‐1 transcription factor activates the expression of mouse UDP‐Gal:GA2/GM2/GD2/GT2 galactosyltransferase gene
Author(s) -
Raimondi Lina P.,
Daniotti Jose L.,
Maccioni Hugo J.F.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.066
Subject(s) - galactosyltransferase , transcription factor , chemistry , gene expression , microbiology and biotechnology , gene , biology , biochemistry , enzyme
UDP‐Gal:GA2/GM2/GD2/GT2 galactosyltransferase (Gal‐T2) transfers galactose to the terminal N‐acetylgalactosamine of either the neutral glycolipid GA2 or of the gangliosides GM2, GD2 and GT2. Previous studies revealed a tight regulation of Gal‐T2 activity and mRNA expression during development of the rat CNS. Here, we study in PC12 cells the cis ‐acting elements involved in the activation of a fragment of 211 bp around the transcription initiation site of the mouse Gal‐T2 promoter. Mutagenesis, competition experiments and functional assays showed that the Ets‐1 transcription factor is involved in the activation of the Gal‐T2 promoter.