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Dual signaling by mGluR5a results in bi‐directional modulation of N‐type Ca 2+ channels
Author(s) -
Sánchez-Prieto J.,
Paternain A.V.,
Lerma J.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.053
Subject(s) - staurosporine , downregulation and upregulation , protein kinase c , metabotropic glutamate receptor , glutamate receptor , stimulation , xenopus , microbiology and biotechnology , chemistry , receptor , biophysics , biology , neuroscience , signal transduction , biochemistry , gene
We have studied how N‐type Ca 2+ channels are modulated by the metabotropic glutamate receptor 5a (mGluR5a) in Xenopus oocytes . Stimulation of the receptor with glutamate initiated two parallel responses, a rapid inhibition followed by an upregulation of the Ca 2+ current. Although a subsequent stimulation did not upregulate the Ca 2+ current, it did still produce a reduction in the amplitude of the current. The upregulation of Ca 2+ channels was prevented by the protein kinases inhibitor staurosporine and it was mimicked by the activation of PKC with phorbol esters. In contrast, the inhibition of the Ca 2+ current was insensitive to staurosporine. These results show that mGluR5a exerts a bi‐directional influence on Ca 2+ channels, which may explain how group I mGluRs facilitate and inhibit glutamate release at central synapses.