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Calcium inhibits muscle FBPase and affects its intracellular localization in cardiomyocytes
Author(s) -
Gizak A.,
Majkowski M.,
Dus D.,
Dzugaj A.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.050
Subject(s) - fructose 1,6 bisphosphatase , enzyme , chemistry , myocyte , intracellular , subcellular localization , calcium , biochemistry , microbiology and biotechnology , fructose , biology , cytoplasm , organic chemistry
As our recent investigation revealed, in mammalian heart muscle, fructose 1,6‐bisphosphatase (FBPase) – a key enzyme of glyconeogenesis – is located around the Z‐line, inside cells' nuclei and, as we demonstrate here for the first time, it associates with intercalated discs. Since the degree of association of numerous enzymes with subcellular structures depends on the metabolic state of the cell, we studied the effect of elevated Ca 2+ concentration on localization of FBPase in cardiomyocytes. In such conditions, FBPase dissociated from the Z‐line, but no visible effect on FBPase associated with intercalated discs or on the nuclear localization of the enzyme was observed. Additionally, Ca 2+ appeared to be a strong inhibitor of muscle FBPase.