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Expression of a cascading genetic network within liposomes
Author(s) -
Ishikawa Keitaro,
Sato Kanetomo,
Shima Yasufumi,
Urabe Itaru,
Yomo Tetsuya
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.046
Subject(s) - liposome , green fluorescent protein , t7 rna polymerase , biology , transfection , microbiology and biotechnology , genetic network , computational biology , chemistry , gene , biochemistry , escherichia coli , bacteriophage
Liposomes have long been used as possible compartments for artificial cells, and it has been shown that liposomes can sustain various types of biochemical reactions. To elevate the degree of molecular complexity of the system in liposomes, we have constructed a two‐stage genetic network encapsulated in liposomes. This two‐stage genetic network was constructed with the plasmid pTH, in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP). We show that the two‐stage genetic network constructed in a cell‐free expression system is functional within liposomes.