Premium
GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori
Author(s) -
Hayakawa Tohru,
Shitomi Yasuyuki,
Miyamoto Kazuhisa,
Hori Hidetaka
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.029
Subject(s) - cry1ac , bacillus thuringiensis , bombyx mori , chemistry , biology , biochemistry , genetically modified crops , transgene , bacteria , gene , genetics
Bombyx mori (Shunrei × Shogetsu) is sensitive to Cry1Aa and resistant to Cry1Ac, both insecticidal proteins of Bacillus thuringiensis . Cry1Aa passed through the peritrophic membrane (PM) much faster (0.37 μg/mm 2 PM/h) than Cry1Ac (0.05 μg/mm 2 PM/h) during the initial observation period. Both Cry1Aa and Cry1Ac bound to the PM but only the binding of Cry1Ac was specifically inhibited by N ‐acetylgalactosamine (GalNAc). When Cry1Ac was pretreated with GalNAc, Cry1Ac permeated the PM much faster. These results suggested that Cry1Ac bound a PM protein via GalNAc on a sugar side chain. The role of the PM on Cry1Ac resistance of B. mori was briefly discussed.