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Transcriptomic responses to cadmium in the ectomycorrhizal fungus Paxillus involutus
Author(s) -
Jacob Christophe,
Courbot Mikaël,
Martin Francis,
Brun Annick,
Chalot Michel
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.028
Subject(s) - paxillus involutus , cadmium , biology , metallothionein , biochemistry , transcriptome , ectomycorrhiza , gene , chemistry , mycorrhiza , gene expression , genetics , bacteria , organic chemistry , symbiosis
The molecular mechanisms underlying the response of ectomycorrhizal fungi to heavy metals in general and cadmium in particular remain poorly understood. We screened 2040 arrayed cDNAs of the ectomycorrhizal fungus Paxillus involutus to identify cadmium‐responsive genes by using differential hybridization. Forty nine (2.4%) of the 2040 cDNAs were differentially expressed, among which transcripts coding a laccase, an aconitase, and a metallothionein were upregulated by 3.9‐, 3.7‐ and 2.8‐fold, respectively, whereas genes coding hydrophobins and threonine dehydratase were strongly downregulated. Our results suggest that complexation of cadmium by phenolic compounds, or by complexing peptides such as metallothioneins, is probably key determinant of the cellular response to cadmium in P. involutus . In addition, the present study suggests that the synthesis of hydrophobins may be efficiently reduced, thus redirecting Cys to the manufacture of Cys‐enriched compounds.