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Identification of genes regulated by oleic acid in Jurkat cells by suppressive subtractive hybridization analysis
Author(s) -
Martins de Lima Thais,
de Oliveira Rodrigues Leonardo,
Henrique Bengtson Mario,
Cleide Sogayar Mari,
Nogueira Alves Bezerra Camila,
Amaral Rebouças Nancy,
Curi Rui
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.025
Subject(s) - jurkat cells , suppression subtractive hybridization , oleic acid , microbiology and biotechnology , gene , biology , gene expression , heat shock protein , cell culture , biochemistry , chemistry , t cell , immune system , genetics , cdna library
In this study, the effect of oleic acid (50 μM) on gene expression of Jurkat cells (human T lymphocytes cell line) was examined using the suppressive subtractive hybridization approach. This technique allowed us to identify genes with higher or lower expression after cell treatment with oleic acid as compared to untreated cells. Oleic acid upregulated the expression of the translation elongation factor alpha 1 and ATP synthase 8 and downregulated gp96 (human tumor rejection antigen gp96), heat‐shock protein 60 and subtilisin‐like protein 4. These results suggest that oleic acid, at plasma physiological concentration, can regulate the expression of important genes to maintain the machinery that ensures cell functioning.