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Fourier transform infrared microspectroscopy as a new tool for nematode studies
Author(s) -
Ami Diletta,
Natalello Antonino,
Zullini Aldo,
Doglia Silvia M.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.022
Subject(s) - nematode , infrared , infrared spectroscopy , caenorhabditis elegans , fourier transform infrared spectroscopy , fourier transform , biology , absorption (acoustics) , identification (biology) , analytical chemistry (journal) , chemistry , computational biology , biophysics , optics , genetics , chromatography , physics , gene , botany , ecology , organic chemistry , quantum mechanics
We report the results of a microspectroscopy study on the Fourier transform infrared (FT‐IR) absorption spectra of Caenorhabditis elegans , collected from the different parts of a single intact specimen – pharynx, intestine and tail regions. The principal absorption bands were assigned to the molecular species present in C. elegans , with an excellent reproducibility for the pharynx spectrum. These results enabled us to explore if FT‐IR microspectroscopy could offer a new tool for nematode identification. As an example, the discrimination among four well characterised nematode taxa is reported. The FT‐IR results completely match those obtained by Blaxter and colleagues through molecular biology [Nature 392 (1998) 71].