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Heterologously expressed protein phosphatase calcineurin downregulates plant plasma membrane H + ‐ATPase activity at the post‐translational level
Author(s) -
Marı́n-Manzano Mari Carmen,
Rodrı́guez-Rosales Maria Pilar,
Belver Andrés,
Donaire Juan Pedro,
Venema Kees
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.008
Subject(s) - calcineurin , phosphatase , yeast , biochemistry , atpase , transgene , chemistry , membrane , in vivo , microbiology and biotechnology , biology , enzyme , gene , medicine , transplantation
To investigate the effects of calcineurin expression on cellular ion homeostasis in plants, we have obtained a transgenic cell culture of tomato, expressing constitutively activated yeast calcineurin. Transgenic cells exhibited reduced growth rates and proton extrusion activity in vivo. We show that reduction of plasma membrane H + ‐ATPase activity by expression of calcineurin is the basis for the observed phenotypes. Transgenic calli and cell suspensions displayed also increased salt tolerance and contained slightly higher Ca 2+ and K + levels. This demonstrates that calcineurin can modulate ion homeostasis in plants as it does in yeast by affecting the activity of primary ion transporters.