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Indole‐3‐carbinol activates the cyclin‐dependent kinase inhibitor p15 INK4b gene
Author(s) -
Matsuzaki Youichirou,
Koyama Makoto,
Hitomi Toshiaki,
Kawanaka Mayumi,
Sakai Toshiyuki
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.09.002
Subject(s) - cyclin dependent kinase , cdk inhibitor , hacat , kinase , cell cycle , cyclin dependent kinase 2 , chemistry , cyclin , cyclin dependent kinase 4 , cyclin d , microbiology and biotechnology , cancer research , biology , gene , biochemistry , in vitro
Indole‐3‐carbinol (I3C) is a naturally occurring compound found in vegetables such as broccoli and cauliflower, and has been shown to arrest human tumor cells in the G1 phase of the cell cycle. However, the molecular mechanism responsible for this effect has not been sufficiently elucidated. We report here that I3C activates the cyclin‐dependent kinase (CDK) inhibitor p15 INK4b gene through its promoter, accompanied by cell growth inhibition in HaCaT cells. Treatment with I3C almost did not affect the expressions of the other CDK inhibitors such as p19 INK4d , p21 WAF1 and p27 Kip1 . These results suggest that p15 INK4b is an important molecular target of I3C among CDK inhibitors.