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Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism
Author(s) -
Franceschetti Marina,
Perry Barry,
Thompson Benjamin,
Hanfrey Colin,
Michael Anthony J.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.08.085
Subject(s) - polyamine , proteomics , transgene , gene isoform , biology , genetically modified mouse , biochemistry , microbiology and biotechnology , gene
Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S ‐adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR‐1, a known marker for systemic acquired resistance.