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Translin associated protein X is essential for cellular proliferation
Author(s) -
Yang Shicheng,
Hecht Norman B.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.08.082
Subject(s) - small hairpin rna , biology , microbiology and biotechnology , transfection , hela , gene , intron , rna , gene expression , green fluorescent protein , cell growth , rna interference , cell culture , genetics
DNA vectors that express short hairpin RNAs (shRNAs) provide a new tool for reverse genetic analysis for selective long‐term reduction of gene expression in mammalian cells. Using shRNA constructs with a cytomegalovirus promoter and an actin intron between the hairpins for stabilization, we reduce expression of an exogenously expressed gene, GFP and the endogenous protein, Translin‐associated factor X (TRAX), in stably transfected Hela cell lines. The reduction of TRAX in Hela cells causes reduced cell proliferation. This decrease is specific as there is no equivalent reduction of the TRAX interacting protein, Testis brain RNA‐binding protein, or any significant increase in a number of interferon‐related target genes.