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Protein A – a new ligand for human C‐reactive protein
Author(s) -
Das Tanusree,
Mandal Chhabinath,
Mandal Chitra
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.08.072
Subject(s) - phosphorylcholine , ligand (biochemistry) , chemistry , c reactive protein , binding protein , protein a , protein ligand , plasma protein binding , surface protein , acute phase protein , biochemistry , calcium binding protein , calcium , biology , receptor , immunology , inflammation , gene , antibody , virology , organic chemistry
Phosphorylcholine (PC) is a classical ligand of C‐reactive protein (CRP), a clinically important acute phase protein. In search of new ligands, CRPs were affinity‐purified from several pathological samples, which exhibited distinct molecular variants induced in different diseases. Both glycosylated and non‐glycosylated CRPs showed calcium‐independent differential‐binding to Staphylococcus aureus cell‐surface Protein A. CRP possesses separate binding sites for Protein A and PC with different binding constants. We have demonstrated that Protein A is another ligand in addition to PC establishing an extended definition of CRP. Protein A binding may impart immunomodulatory roles of CRP in combating microorganisms or other foreign materials.