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Laccase from Melanocarpus albomyces binds effectively to cellulose
Author(s) -
Kiiskinen Laura-Leena,
Palonen Hetti,
Linder Markus,
Viikari Liisa,
Kruus Kristiina
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.08.040
Subject(s) - laccase , cellulose , cellulase , chemistry , thermophile , binding site , microcrystalline cellulose , adsorption , nuclear chemistry , biochemistry , enzyme , organic chemistry
A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one‐site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non‐specific protein or 0.1–0.5 M Na 2 SO 4 . No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.