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WD repeat‐containing mitotic checkpoint proteins act as transcriptional repressors during interphase
Author(s) -
Yoon Young-Mee,
Baek Kwan-Hyuck,
Jeong Sook-Jung,
Shin Hyun-Jin,
Ha Geun-Hyoung,
Jeon Ae-Hwa,
Hwang Sang-Gu,
Chun Jang-Soo,
Lee Chang-Woo
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.089
Subject(s) - microbiology and biotechnology , anaphase promoting complex , mad2 , cdc20 , biology , kinetochore , cell cycle checkpoint , anaphase , genetics , cell cycle , gene , chromosome
WD repeats are implicated in protein–protein interactions and regulate a wide variety of cellular functions, including chromatin remodeling and transcription. The WD repeats of the Bub3 and Cdc20 kinetochore proteins are important for the physical interactions of these proteins with Mad2 and BubR1 to yield a kinetochore protein complex capable of delaying anaphase by inhibiting ubiquitin ligation via the anaphase‐promoting complex/cyclosome. Here, we show that Bub3 and Cdc20 form a complex with histone deacetylases; this interaction appears to confer transcriptional repressor activity in a heterologous DNA‐binding context. In addition, inhibition of Bub3 and Cdc20 expression significantly impairs interphase cell cycle. These results indicate that Bub3 and Cdc20 play additional roles in the integration of cell cycle arrest as transcriptional repressors.