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Genome based identification and analysis of the pre‐replicative complex of Arabidopsis thaliana
Author(s) -
Masuda H.P.,
Ramos G.B.A.,
de Almeida-Engler J.,
Cabral L.M.,
Coqueiro V.M.,
Macrini C.M.T.,
Ferreira P.C.G.,
Hemerly A.S.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.088
Subject(s) - arabidopsis , biology , arabidopsis thaliana , origin recognition complex , dna replication factor cdt1 , genome , genetics , gene , origin of replication , dna replication , dna , computational biology , eukaryotic dna replication , microbiology and biotechnology , mutant
Eukaryotic DNA replication requires an ordered and regulated machinery to control G1/S transition. The formation of the pre‐replicative complex (pre‐RC) is a key step involved in licensing DNA for replication. Here, we identify all putative components of the full pre‐RC in the genome of the model plant Arabidopsis thaliana . Different from the other eukaryotes, Arabidopsis houses in its genome two putative homologs of ORC1 , CDC6 and CDT1 . Two mRNA variants of AtORC4 subunit, with different temporal expression patterns, were also identified. Two‐hybrid binary interaction assays suggest a primary architectural organization of the Arabidopsis ORC, in which AtORC3 plays a central role in maintaining the complex associations. Expression profiles differ among pre‐RC components suggesting the existence of various forms of the complex, possibly playing different roles during development. In addition, the expression of the putative pre‐RC genes in non‐proliferating plant tissues suggests that they might have roles in processes other than DNA replication licensing.