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Tumour necrosis factor alpha regulates iron transport and transporter expression in human intestinal epithelial cells
Author(s) -
Johnson Deborah,
Bayele Henry,
Johnston Kelly,
Tennant Jason,
Srai Surjit Kaila,
Sharp Paul
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.081
Subject(s) - dmt1 , tumor necrosis factor alpha , transporter , cytokine , chemistry , microbiology and biotechnology , inflammation , proinflammatory cytokine , metabolism , caco 2 , endocrinology , biology , medicine , biochemistry , immunology , cell , gene
TNFα has dramatic effects on iron metabolism contributing to the generation of hypoferraemia in the anaemia of chronic disease. Interestingly, TNFα is also synthesised and released within the intestinal mucosa, suggesting that this pro‐inflammatory cytokine may play a role in regulating dietary iron absorption. To investigate this possibility, we stimulated intestinal Caco‐2 cells with TNFα (10 ng/ml). In TNFα‐treated cells, apical iron uptake was significantly decreased and this was accompanied by a reduction in divalent metal transporter protein and mRNA expression. Our data suggest that TNFα could regulate dietary iron absorption and that the apical transport machinery is the target for these actions.