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Control of specific gene expression in mammalian cells by co‐expression of long complementary RNAs
Author(s) -
Tran Nham,
Raponi Mitch,
Dawes Ian W.,
Arndt Greg M.
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.075
Subject(s) - gene silencing , dicer , gene expression , biology , rna silencing , gene , regulation of gene expression , rna interference , microbiology and biotechnology , rna induced silencing complex , rna , embryonic stem cell , trans acting sirna , genetics
The use of long double‐stranded RNA (dsRNA) for gene silencing in mammalian cells has generally been restricted to embryonic cell types and proposed to induce non‐specific effects on gene expression in differentiated cells. In this study, we report that foreign and endogenous gene expression can be regulated in immortalised human cell lines by co‐expression of long complementary RNAs with the potential to form dsRNA. The observed gene silencing effect was transferable to recipient control cells, occurred independently of cytoplasmic Dicer and produced an epi‐allelic series of clones suitable for gene function studies. This complementary RNA co‐expression approach permits the use of long complementary RNAs for regulating specific gene expression in mammalian cells.