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Identification of region‐specific transcription factor genes in the adult mouse brain by medium‐scale real‐time RT‐PCR
Author(s) -
Suzuki Harukazu,
Okunishi Rie,
Hashizume Wataru,
Katayama Shintaro,
Ninomiya Noriko,
Osato Naoki,
Sato Kenjiro,
Nakamura Mari,
Iida Juri,
Kanamori Mutsumi,
Hayashizaki Yoshihide
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.068
Subject(s) - gene , biology , transcription factor , real time polymerase chain reaction , transcription (linguistics) , messenger rna , gene expression , reverse transcription polymerase chain reaction , identification (biology) , genetics , computational biology , microbiology and biotechnology , philosophy , linguistics , botany
We established a medium-scale real-time RT-PCR system focusing on transcription factors and applied it to their expression profiles in the adult mouse 11 brain regions (http://genome.gsc.riken.jp/qRT-PCR/). Almost 90% of the examined genes showed significant expression in at least one region. We successfully extracted 179 region-specific genes by clustering analysis. Interestingly, the transcription factors involved in the development of the pituitary were still expressed in the adult pituitary, suggesting that they also play important roles in maintenance of the pituitary. These results provide unique molecular markers that may account for the molecular basis of the unique functions of specific brain regions.