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In Arabidopsis thaliana , the invertase inhibitors AtC/VIF1 and 2 exhibit distinct target enzyme specificities and expression profiles
Author(s) -
Link Manuela,
Rausch Thomas,
Greiner Steffen
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.062
Subject(s) - invertase , arabidopsis thaliana , mutant , biology , biochemistry , arabidopsis , enzyme , sugar , microbiology and biotechnology , gene
Plant cell wall (CWI) and vacuolar invertases (VI) play important roles in carbohydrate metabolism, stress responses and sugar signaling. Addressing the regulation of invertase activities by inhibitor proteins (C/VIF, ell wall/ acuolar nhibitor of ructosidase), we have identified two C/VIFs from Arabidopsis thaliana . AtC/VIF1 showed specific inhibition of VI activity, whereas AtC/VIF2 inhibited both, CWI and VI. Expression analysis revealed that expression of AtC/VIF1 was restricted to specific organs, AtC/VIF2, however, was weakly expressed throughout plant development. Promoter::GUS transformants confirmed pronounced differences of tissue/cell type‐specific expression between both isoforms. Growth of an AtC/VIF1 T‐DNA KO mutant was unaffected, but VI activity and hexose content were slightly increased.