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bFGF rescues 423‐cells from serum starvation‐induced apoptosis downstream of activated caspase‐3
Author(s) -
Schamberger Chantal J.,
Gerner Christopher,
Cerni Christa
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.053
Subject(s) - starvation , apoptosis , microbiology and biotechnology , downstream (manufacturing) , chemistry , caspase 8 , caspase 3 , caspase , biology , programmed cell death , biochemistry , endocrinology , business , marketing
Serum withdrawal rapidly induces apoptosis in rat 423‐cells, while addition of bFGF results in cell survival. However, surviving cells initially display morphological changes characteristic for apoptotic cells and even process caspases. Active caspase‐3 was detected at the single‐cell level in those finally bFGF‐rescued cells, while mitochondrial integrity was maintained. Generation of cleavage products of caspase targets was confirmed in surviving cells. Proteome analysis indicated multi‐faceted survival activities of bFGF including upregulation of inhibitor‐of‐apoptosis and heat shock protein family members directly interfering with caspases. Our data suggest that the “point‐of‐no‐return” in death‐induced cells has to be moved downstream of activated caspase‐3.