Non‐enzymatic platelet‐activating factor formation by acetylated proteins | Zendy

Thanou Paraskevi G. | Zendy; Tsoukatos Demokritos C. | Zendy

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Non‐enzymatic platelet‐activating factor formation by acetylated proteins

Author(s) -

Thanou Paraskevi G.,

Tsoukatos Demokritos C.

Publication year - 2004

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2004.07.037

Subject(s) - acetylation , platelet activating factor , chemistry , phospholipid , bovine serum albumin , biochemistry , phosphocholine , enzyme , incubation , albumin , lipoprotein , phosphatidylcholine , cholesterol , biology , endocrinology , membrane , gene

Substantial amounts of platelet‐activating factor (PAF 1‐ O ‐alkyl‐2‐acyl‐sn‐glycero‐3‐phosphocholine), the potent phospholipid mediator of allergic and inflammatory reactions, are formed upon incubation of acetylated low‐density lipoprotein, acetylated bovine serum albumin (BSA) and acetylated apolipoprotein A‐I with 1‐0‐hexadecyl‐sn‐glycero‐3‐phosphocholine (lyso‐PAF). Acetylated BSA produced 0.3 nmol PAF/mg of protein after a 6 h incubation period with 40 μM lyso‐PAF. The transfer of acetate bound to acetylated proteins to lyso‐PAF was non‐enzymatic. Chemical PAF formation by acetylated proteins, involved in lipid metabolism and transport, could lead to complication of inflammatory and allergic events.

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