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β‐Dystroglycan can be revealed in microsomes from mdx mouse muscle by detergent treatment
Author(s) -
Cluchague Nicolas,
Moreau Céline,
Rocher Chantal,
Pottier Sandrine,
Leray Geneviève,
Cherel Yan,
Le Rumeur Elisabeth
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.032
Subject(s) - dystroglycan , dystrophin , skeletal muscle , utrophin , transmembrane protein , muscular dystrophy , mdx mouse , membrane protein , duchenne muscular dystrophy , chemistry , microbiology and biotechnology , itga7 , medicine , biology , biochemistry , membrane , endocrinology , receptor , laminin , extracellular matrix
β‐Dystroglycan is the central member of a transmembrane protein complex of the skeletal muscle plasma membrane. Since it was not detected in dystrophin‐deficient skeletal muscles, a disruption of the complex was thought to be involved in the dystrophic process. We report here that β‐dystroglycan is actually present at normal levels in mdx mouse muscle plasma membrane: treatment with cholate detergent is able to reveal its presence by SDS–PAGE and immunoblotting. This result shows that, in dystrophin‐deficient muscles, β‐dystroglycan is indeed targeted to the plasma membrane but remains inaccessible to classical solubilizing treatments and to antibodies used for immunolocalization.