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A short autonomous repression motif is located within the N‐terminal domain of CTCF
Author(s) -
Drueppel Lars,
Pfleiderer Klaus,
Schmidt Andreas,
Hillen Wolfgang,
Berens Christian
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.027
Subject(s) - psychological repression , repressor , enhancer , ctcf , sequence motif , motif (music) , biology , gene silencing , transcription (linguistics) , transcription factor , genetics , microbiology and biotechnology , gene , gene expression , linguistics , physics , philosophy , acoustics
The vertebrate transcription factor CTCF is not only involved in transcriptional activation, insulation and genomic imprinting, but also in transcriptional repression. Sequence motifs mediating these activities have not been identified so far. We have mapped a short repression motif to residues 150–170 within the N‐terminal domain of CTCF. This motif is active in HeLa, HEK293 and COS‐7 cell lines where it is both sufficient and necessary for silencing either an SV40‐, or a CMV‐enhancer. It also represses the basal activity of an SV40 core promoter. Since this autonomous repression motif displays no sequence similarity to any other regulatory protein, it represents a yet unknown co‐repressor recruiting motif.