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Identification and characterization of the nuclear import and export signals of the mammalian Ste20‐like protein kinase 3
Author(s) -
Lee Wan-Shu,
Hsu Chiung-Yueh,
Wang Pei-Ling,
Huang Chi-Ying Fred,
Chang Chia-Hua,
Yuan Chiun-Jye
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.007
Subject(s) - nls , nuclear localization sequence , cleavage (geology) , nuclear export signal , microbiology and biotechnology , protein kinase a , cytoplasm , subcellular localization , kinase , biology , cell nucleus , paleontology , fracture (geology)
Mst3, a human Ste20‐like protein kinase, has been recently demonstrated to undergo a caspase‐mediated cleavage during apoptosis. The proteolytic cleavage of the C‐terminus of Mst3 caused nuclear translocation of its kinase domain. This work provides evidence that Mst3 may contain a bipartite‐like nuclear localization sequence (NLS) at the C‐terminus of its kinase domain (residues 278–292). The removal of NLS from the kinase domain of Mst3 led to the cytoplasmic accumulation of EGFP‐Mst3 Δ277 . The presence of nuclear exporting signals in the Mst3 was also demonstrated by leptomycin B‐treatment and serial deletion of the C‐terminal regulatory domain of Mst3. A nuclear export signal was also postulated to be in the regions of amino acids 335–386. In conclusion, Mst3 contains both NLS and NES signals, which may cooperate to control the subcellular distribution of Mst3.