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Stemar‐13‐ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice
Author(s) -
Nemoto Tadahiro,
Cho Eun-Min,
Okada Atsushi,
Okada Kazunori,
Otomo Kazuko,
Kanno Yuri,
Toyomasu Tomonobu,
Mitsuhashi Wataru,
Sassa Takeshi,
Minami Eiichi,
Shibuya Naoto,
Nishiyama Makoto,
Nojiri Hideaki,
Yamane Hisakazu
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.07.002
Subject(s) - phytoalexin , elicitor , diterpene , cyclase , escherichia coli , biochemistry , biology , biosynthesis , terpenoid , complementary dna , atp synthase , enzyme , gene , resveratrol
In suspension‐cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors. We isolated a cDNA encoding a diterpene cyclase, OsDTC2, from suspension‐cultured rice cells treated with a chitin elicitor. The OsDTC2 cDNA was overexpressed in Escherichia coli as a fusion protein with glutathione S ‐transferase, and the recombinant OsDTC2 was indicated to function as stemar‐13‐ene synthase that converted syn ‐copalyl diphosphate to stemar‐13‐ene, a putative diterpene hydrocarbon precursor of the phytoalexin oryzalexin S. The level of OsDTC2 mRNA in suspension‐cultured rice cells began to increase 3 h after addition of the elicitor and reached the maximum after 8 h. The expression of OsDTC2 was also induced in UV‐irradiated rice leaves. In addition, we indicated that stemar‐13‐ene accumulated in the chitin‐elicited suspension‐cultured rice cells and the UV‐irradiated rice leaves.