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Cloning and characterization of a Streptomyces antibioticus ATCC11891 cyclophilin related to Gram negative bacteria cyclophilins
Author(s) -
Manteca Angel,
Kamphausen Thilo,
Fanghanel Jorg,
Fischer Gunter,
Sanchez Jesus
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.091
Subject(s) - cyclophilin , cyclophilin a , bacteria , cloning (programming) , microbiology and biotechnology , gram negative bacteria , gram , chemistry , biochemistry , biology , escherichia coli , genetics , gene , computer science , programming language
Cyclophilins are folding helper enzymes and represent a family of the enzyme class of peptidyl‐prolyl cis–trans isomerases. Here, we report the molecular cloning and biochemical characterization of San Cyp18, an 18‐kDa cyclophilin from Streptomyces antibioticus ATCC11891 located in the cytoplasm and constitutively expressed during development. Amino acid sequence analysis revealed a much higher homology to cyclophilins from Gram negative bacteria than to known cyclophilins from Streptomyces or other Gram positive bacteria. San Cyp18 is inhibited weakly by CsA, with a K i value of 21 μM, similar to cyclophilins from Gram negative bacteria. However, this value is more than 20‐fold higher than the K i values reported for cyclophilins from other Gram positive bacteria, which makes San Cyp18 unique within this group. The presence of San Cyp18 in Streptomyces is likely due to horizontal gene transmission from Gram‐negative bacteria to Streptomyces .