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INFγ stimulates arginine transport through system y + L in human monocytes
Author(s) -
Rotoli Bianca Maria,
Bussolati Ovidio,
Sala Roberto,
Barilli Amelia,
Talarico Enrica,
Gazzola Gian C,
Dall'Asta Valeria
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.086
Subject(s) - arginine , protein subunit , microbiology and biotechnology , chemistry , monocyte , gene , biochemistry , biology , amino acid , immunology
Freshly isolated human monocytes transport l ‐arginine mostly through a sodium independent, NEM insensitive pathway inhibited by l ‐leucine in the presence, but not in the absence of sodium. Interferon‐γ (IFNγ) stimulates this pathway, identifiable with system y + L, and markedly enhances the expression of SLC7A7, the gene that encodes for system y + L subunit y+LAT1, but not of SLC7A6, that codes for the alternative subunit y+LAT2. System y + plays a minor role in arginine uptake by monocytes and the expression of system y + ‐related genes, SLC7A1 and SLC7A2, is not changed by IFNγ. These results demonstrate that system y + L is sensitive to IFNγ.