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Specificity determinants for chemokine recognition identified using eotaxin‐MCP‐1 chimeras
Author(s) -
Mayer Michael R,
Parody Todd R,
Datta-Mannan Amita,
Stone Martin J
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.079
Subject(s) - eotaxin , ccr3 , chemokine receptor , ccr2 , ccr10 , chemokine , cc chemokine receptors , ccl7 , c c chemokine receptor type 6 , ccl13 , ccr1 , xcl2 , receptor , ccl21 , microbiology and biotechnology , ccl25 , biology , cxcl2 , chemistry , biochemistry
To identify the elements of two chemokines [monocyte chemoattractant protein‐1 (MCP‐1) and eotaxin] that control their differential recognition by their respective receptors (CCR2 and CCR3), we have studied the receptor interactions of MCP‐1‐eotaxin chimeras. Each receptor was found to exhibit a distinct binding preference for proteins containing the amino‐terminal region of the cognate chemokine for that receptor. However, other elements dictating chemokine preference were different for the two receptors. In some cases, the influence of replacing a particular region was dependent on the identities of neighboring regions, indicating a complex network of cooperative and/or compensating interactions.