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Staurosporine restores GTPγS induced block of rapid endocytosis in chromaffin cells
Author(s) -
Henkel Andreas Wolfram,
Vogt Tobias Kristian,
Henkel Maria Kerstin
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.069
Subject(s) - endocytosis , gtpgammas , staurosporine , exocytosis , chemistry , microbiology and biotechnology , bulk endocytosis , intracellular , biophysics , gtp' , receptor mediated endocytosis , membrane , biochemistry , biology , protein kinase c , receptor , signal transduction , enzyme
Fast capacitance measurements demonstrated that chromaffin cells retrieve membrane by several kinetically different pathways. Here, we show that rapid endocytosis is blocked and slow endocytosis reduced by intracellular application of GTPγS, an activator of G‐proteins, but not by the competitive blocker GDPβS. The inhibition of rapid endocytosis by GTPγS can be restored with GDPβS or staurosporine completely. But only staurosporine partially abolishes the reduction of slow endocytosis by GTPγS. Besides triggering exocytosis, GTPγS elicits large exo‐ and endocytotic vesicles that contributed significantly to the total membrane traffic, indicating a third pathway of membrane shuttle.