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Inhibitory action of novel aromatic diamine compound on lipopolysaccharide‐induced nuclear translocation of NF‐κB without affecting IκB degradation
Author(s) -
Shin Hyun-Mo,
Kim Min-Hee,
Kim Byung Hak,
Jung Sang-Hun,
Kim Yeong Shik,
Park Hye Ji,
Hong Jin Tae,
Min Kyung Rak,
Kim Youngsoo
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.056
Subject(s) - lipopolysaccharide , tumor necrosis factor alpha , chemistry , chromosomal translocation , nf κb , nitric oxide , apoptosis , mechanism of action , nitric oxide synthase , biochemistry , inhibitory postsynaptic potential , nfkb1 , microbiology and biotechnology , enzyme , in vitro , biology , endocrinology , transcription factor , gene , organic chemistry
4‐Methyl‐ N 1 ‐(3‐phenyl‐propyl)‐benzene‐1,2‐diamine (JSH‐23) is a novel chemically synthetic compound. The aromatic diamine JSH‐23 compound exhibited inhibitory effect with an IC 50 value of 7.1 μM on nuclear factor (NF)‐κB transcriptional activity in lipopolysaccharide (LPS)‐stimulated macrophages RAW 264.7, and interfered LPS‐induced nuclear translocation of NF‐κB without affecting IκB degradation. This mechanism of action is very rare for controlling NF‐κB activation. Furthermore, the compound inhibited not only LPS‐induced expressions of tumor necrosis factor‐α, interleukin (IL)‐1β, IL‐6 and inducible nitric oxide synthase and cyclooxygenase‐2 but also LPS‐induced apoptosis of the RAW 264.7 cells.