Premium
Identification of O 2 ‐induced peptides in an obligatory anaerobe, Clostridium acetobutylicum
Author(s) -
Kawasaki Shinji,
Ishikura Jun,
Watamura Yusuke,
Niimura Youichi
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.047
Subject(s) - clostridium acetobutylicum , clostridium , identification (biology) , microbiology and biotechnology , chemistry , biochemistry , biology , bacteria , butanol , genetics , ethanol , botany
Clostridium acetobutylicum DSM792 (=ATCC824), a solvent producing obligate anaerobe, grew well after a shift in growth conditions from anoxic to microoxic at the mid exponential phase. In two‐dimensional gel electrophoresis, a spot migrating at 45 kDa and three spots at 23 kDa accumulated after 30 min of flushing with 5% O 2 /95% N 2 . Based on peptide mass fingerprints, the 45 kDa polypeptide was determined to be NP_347663 (A‐type flavoprotein homologue) and the 23 kDa polypeptides were determined to be NP_350180 or NP_350181 (novel type rubrerythrin homologue). Northern blot analysis indicated that the expressions of these peptide transcripts were upregulated within 10 min after flushing with 5% O 2 /95% N 2 .