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Aβ production as consequence of cellular death of a human neuroblastoma overexpressing APP
Author(s) -
Recuero Marı́a,
Serrano Elena,
Bullido Marı́a J,
Valdivieso Fernando
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.025
Subject(s) - neuroblastoma , programmed cell death , cell culture , apoptosis , secretion , peptide , transfection , amyloid precursor protein , microbiology and biotechnology , human brain , cell , biology , p3 peptide , cancer research , chemistry , alzheimer's disease , disease , neuroscience , medicine , biochemistry , genetics
In human brain the Aβ peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Aβ secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which overexpress APP; both of them secreted Aβ and showed morphological changes and cell death with apoptotic program characteristics. Interestingly, coculture experiments with the untransfected human neuroblastoma cell line showed that the Aβ peptide was not responsible for the death in those cell lines; additionally, we indicate that upon cell death, Aβ peptide is secreted into cell medium.