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The African swine fever virus dynein‐binding protein p54 induces infected cell apoptosis
Author(s) -
Hernáez Bruno,
Dı́az-Gil Gema,
Garcı́a-Gallo Mónica,
Ignacio Quetglas José,
Rodrı́guez-Crespo Ignacio,
Dixon Linda,
Escribano José M,
Alonso Covadonga
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.06.001
Subject(s) - dynein , apoptosis , african swine fever virus , microbiology and biotechnology , biology , effector , vero cell , caspase , internalization , programmed cell death , virus , virology , cell , biochemistry , microtubule
A specific interaction of ASFV p54 protein with 8 kDa light chain cytoplasmic dynein (DLC8) has been previously characterized and this interaction is critical during virus internalization and transport to factory sites. During early phases of infection, the virus induces the initiation of apoptosis triggering activation of caspase‐9 and ‐3. To analyze the role of the structural protein p54 in apoptosis, transient expression experiments of p54 in Vero cells were carried out which resulted in effector caspase‐3 activation and apoptosis. Interestingly, p54 mutants, lacking the 13 aa dynein‐binding motif lose caspase activation ability and pro‐death function of p54. This is the first reported ASFV protein which induces apoptosis.