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Recycling of intact dense core vesicles in neurites of NGF‐treated PC12 cells
Author(s) -
Bauer Roslyn A,
Khera Rebecca S,
Lieber Janet L,
Angleson Joseph K
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.086
Subject(s) - endocytosis , vesicle , immunoelectron microscopy , endocytic cycle , neurite , chemistry , microbiology and biotechnology , biophysics , exocytosis , fluorescence microscope , vesicle fusion , chromogranin a , extracellular , synaptic vesicle , membrane , biochemistry , in vitro , fluorescence , biology , immunohistochemistry , immunology , cell , physics , quantum mechanics
Exocytic fusion in neuroendocrine cells does not always result in complete release of the peptide contents from dense core vesicles (DCVs). In this study, we use fluorescence imaging and immunoelectron microscopy to examine the retention, endocytosis and recycling of chromogranin B in DCVs of NGF‐treated PC12 cells. Our results indicate that DCVs retained and retrieved an intact core that was available for subsequent exocytic release. The endocytic process was inhibited by cyclosporine A or by substitution of extracellular Ca 2+ with Ba 2+ and the total recycling time was less than 5 min.
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