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A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells
Author(s) -
Hirano Katsuya,
Hirano Mayumi,
Nishimura Junji,
Kanaide Hideo
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.084
Subject(s) - transduction (biophysics) , signal transduction , microbiology and biotechnology , signaling proteins , extracellular , cell cycle , biology , cell , chemistry , biochemistry
The time‐specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell‐penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time‐specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G 1 –S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time‐specific role of signaling proteins.