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PIASy represses TRIF‐induced ISRE and NF‐κB activation but not apoptosis
Author(s) -
Zhang Jun,
Xu Liang-Guo,
Han Ke-Jun,
Wei Xudong,
Shu Hong-Bing
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.081
Subject(s) - trif , chemistry , sendai virus , apoptosis , nf κb , microbiology and biotechnology , biology , gene , receptor , innate immune system , toll like receptor , biochemistry
The TIR domain‐containing adapter protein TRIP is critically involved in TLR3‐induced IFN‐β production through activation of NF‐κB and ISRE. In addition, TRIF also induces apoptosis when overexpressed in 293 cells. In this report, we demonstrate that PIASy, a member of the PIAS SUMO‐ligase family, interacts with TRIP, IRF‐3 and IRF‐7. In reporter gene assays, PIASy dramatically inhibits TRIF‐induced NF‐κB, ISRE and IFN‐β activation but not TRIF‐induced apoptosis. Furthermore, PIASy also inhibits IRF‐3, IRF‐7 and Sendai virus‐induced ISRE activation. Our results suggest that PIASy is an inhibitor of TRIF‐induced ISRE and NF‐κB activation but not apoptosis.