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Phorbol ester‐induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon‐associating proteins
Author(s) -
Xu Tian-Rui,
Rumsby Martin G
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.080
Subject(s) - protein kinase c , nucleus , vimentin , microbiology and biotechnology , chemistry , immunoprecipitation , cell nucleus , chromosomal translocation , nuclear protein , blot , phorbol , biology , biochemistry , signal transduction , gene , immunology , transcription factor , immunohistochemistry
We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKCε from a perinuclear site to the nucleus, extending findings in PC12 and NG108‐15 cells and in myocytes. We have immunoprecipitated the PKCε from nuclei isolated from phorbol ester‐treated fibroblasts and identified six proteins which associate with nuclear PKCε. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, β‐actin and annexin II by MALDI–TOF–MS. We have confirmed that these proteins associate with PKCε by gel overlay and/or dot blotting assays. The role of these PKCε‐associating proteins in the nucleus and their interaction with PKCε are considered.