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Increase of SUMO‐1 expression in response to hypoxia: direct interaction with HIF‐1α in adult mouse brain and heart in vivo
Author(s) -
Shao Ruijin,
Zhang Fu-Ping,
Tian Fei,
Anders Friberg P,
Wang Xiaoyang,
Sjöland Helen,
Billig Håkan
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.079
Subject(s) - sumo protein , in vivo , hypoxia (environmental) , microbiology and biotechnology , stimulation , transcription factor , immunoprecipitation , biology , in vitro , ubiquitin , hypoxia inducible factors , messenger rna , chemistry , endocrinology , biochemistry , gene , oxygen , genetics , organic chemistry
The present study investigates the regulation of small ubiquitin‐related modifier‐1 (SUMO‐1) expression in response to hypoxia in adult mouse brain and heart. We observed a significant increase in SUMO‐1 mRNAs and proteins after hypoxic stimulation in vivo. Because SUMO‐1 interacts with various transcription factors, including hypoxia‐inducible factor‐1β (HIF‐1β) in vitro, we not only demonstrated that the HIF‐1α expression is increased by hypoxia in brain and heart, but also provided evidence that SUMO‐1 co‐localizes in vivo with HIF‐1α in response to hypoxia by demonstrating the co‐expression of these two proteins in neurons and cardiomyocytes. The specific interaction between SUMO‐1 and HIF‐1α was additionally demonstrated with co‐immunoprecipitation. These results indicate that the increased levels of SUMO‐1 participate in the modulation of HIF‐1α function through sumoylation in brain and heart.