Premium
Enterobacter aerogenes OmpX, a cation‐selective channel mar ‐ and osmo‐regulated
Author(s) -
Dupont Myrielle,
Dé Emmanuelle,
Chollet Renaud,
Chevalier Jacqueline,
Pagès Jean-Marie
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.047
Subject(s) - enterobacter aerogenes , porin , microbiology and biotechnology , bacterial outer membrane , chemistry , biology , escherichia coli , biochemistry , gene
The ompX gene of Enterobacter aerogenes was cloned. Its overexpression induced a decrease in the major porin Omp36 production and consequently a β‐lactam resistance was noted. Purified outer membrane protein X (OmpX) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 pS in 1 M NaCl and a cationic selectivity. Both MarA expression and high osmolarity induced a noticeable increase of the OmpX synthesis in the E. aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non‐specific porins, Omp36 and Omp35, decreased.