Premium
RhoA controls myoblast survival by inducing the phosphatidylinositol 3‐kinase‐Akt signaling pathway
Author(s) -
Reuveny Mickol,
Heller Hanna,
Bengal Eyal
Publication year - 2004
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2004.05.035
Subject(s) - rhoa , protein kinase b , microbiology and biotechnology , signal transduction , kinase , phosphatidylinositol , mapk/erk pathway , phosphorylation , pi3k/akt/mtor pathway , small gtpase , myod , chemistry , biology , cancer research , myocyte , myogenesis
The small GTPase RhoA regulates the expression of the myogenic transcription factor, MyoD, and the transcription of muscle‐specific genes. We report that RhoA also affects the survival of differentiating myoblasts. Two signaling pathways, extracellular signal‐regulated kinase (ERK) and phosphatidylinositol 3‐kinase (PI3‐K)‐Akt, are involved in myoblast survival. Here, we show that inhibition of RhoA prevents the phosphorylation of Akt, but does not affect the phosphorylation of ERK. Constitutive expression of an active form of Akt prevents apoptosis in myoblasts treated with the Rho inhibitor C3‐transferase. These results indicate that RhoA functions to prevent myoblast death by inducing the PI3‐K‐Akt pathway.